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KMID : 0903520090520010011
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 1 p.11 ~ p.16
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Expression of Recombinant Cyclooxygenase 1 in Trichoplusia ni BTI Tn 5B1-4 Cells Transformed with Human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase
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Chang Kyung-Hwa
Yoo Ki-Hyun
Hwangbo-Jeon
Seok Yeon-Ju
Lee Jong-Min
Kim Kyung-Il
Lee Youn-Hyung
Yang Jai-Myung
Baek Nam-In
Chung In-Sik
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Abstract
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Expression of the human cyclooxygenase 1 (COX-1) in Trichoplusia ni BTI Tn 5B1-4 cells transformed with cDNAs encoding β1,4-galactosyltransferase (GalT) and Galβ1,4-GlcNAc α2,6-sialyltransferase (ST6Gal) was examined. Southern blot analysis indicated that the glycosyltransferase genes were integrated into the Tn 5B1-4 cell genome. A lectin blot analysis also indicated that the recombinant COX-1s from the Tn 5B1-4/COX-1/GalT-ST cells contained the glycan residues of β1,4-linked galactose and α2,6-linked sialic acid. The specific peroxidase activity of the recombinant sialylated COX-1 from Tn 5B1-4/COX-1/GalT-ST cells was 23,930 units/mg, indicating an increase of approximately 17% compared with the non-sialylated control (20,500 units/mg) from the Tn 5B1-4/COX-1 cells.
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KEYWORD
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recombinant cyclooxygenase 1, Trichoplusia ni BTI Tn 5B1-4 cells, β1, 4-galactosyltransferase, Galβ1, 4-GlcNAc α2, 6-sialyltransferase
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